Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/05/01

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Overview

Plates were incubated at 37°C overnight, then colonies were counted.

Sample # colonies
KAH57 >100
KAH104 >100
pMaxGFP >500
KAH57 & KAH104 (BB cloning) >100
BB cloning control (digested KAH57) 0
neg control (water) 0

Looks like everything went as planned. Will start liquid cultures next week.