Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/28

Transformation from DBN004_pSB1A3 Ligation

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Overview I'll be checking the plates for colonies and starting liquid cultures & plates from the samples if colonies appear. Transformation Results Plate # colonies neg. control 0 colony 1 10 colony 2 12 colony 5 4 Liquid Cultures & Streaks Four colonies were picked from each transformation plate. They're numbered 1.1, 1.2, 1.3, 1.4, 2.1 etc. where the first number is the original (source) colony and the second number is the colony picked today. There are twelve colonies picked in total, streaked on a library plate and placed in 5mL aliquots of LB + Amp.