Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/15
|Transformation Results for DBN001_pSB1A3||Main project page|
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Here are the colonies observed after one weekend grown at room temperature. I'll also be doing a plasmid miniprep of mCh_pSB1A3 so that I have more stocks of the cloning vector.
I'm going to set up two cultures for the two colonies on the sample plate, but am not very confident of the success of the ligation given the colonies on the negative control.
mCh_pSB1A3 Plasmid Miniprep
Decent yield for all four colonies picked.
Digest for 15 minutes at 37°C.
Expected size fragments from EcoRI/PstI digest: 2114, 980