Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/07

DBN002 Gel Purification	Main project page Previous entry      Next entry
Overview I took the 55°C and 56°C fractions from the thermal gradient PCR of DBN002 with f2 primer (here) and ran them on a 1% agarose gel, then excised the 1444bp fragment and purified it using the GenElute gel extraction kit. DNA concentration (n=2): Sample ID average ng/µL stdev ng/µL DBN002 16.84 1.261 Yield is low, but should be sufficient for Type IIS ligation.