Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/07

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Overview

I took the 55°C and 56°C fractions from the thermal gradient PCR of DBN002 with f2 primer (here) and ran them on a 1% agarose gel, then excised the 1444bp fragment and purified it using the GenElute gel extraction kit.

DNA concentration (n=2):

Sample ID average ng/µL stdev ng/µL
DBN002 16.84 1.261

Yield is low, but should be sufficient for Type IIS ligation.