Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/07
|DBN002 Gel Purification||Main project page|
Previous entry Next entry
I took the 55°C and 56°C fractions from the thermal gradient PCR of DBN002 with f2 primer (here) and ran them on a 1% agarose gel, then excised the 1444bp fragment and purified it using the GenElute gel extraction kit.
DNA concentration (n=2):
Yield is low, but should be sufficient for Type IIS ligation.