Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/03/19

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Overview

Today I ran an agarose gel on the PCR products created yesterday. Gel layout is as follows:

1kb Plus Ladder DBN002 DBN002 (-) DBN003 DBN003 (-)

Samples indicated with (-) are negative controls. Gel image below:

2015-03-19 PCR products v2.png

The DBN002 reaction appears to have failed completely, as no band is visible (expected fragment size is ~1500bp). The DBN003 reaction was a success, with a >500bp fragment visible in its lane. Expected size of the fragment is 563bp. No bands are visible in the negative controls.