Reagent
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Volume
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Expected:
BL01/5 Ligation Colonies [XbaI/NruI]
Forward insertion: 3659, 4064
Reverse insertion: 1538, 6185
No insertion: 1538, 4064
CMV/MV9 with [XbaI/NruI]
1538, 4064
Uncut CMV/MV9: 5197
Uncut CMV/BL05/MV9: 7723
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15 μL/lane; 1% agarose;
Observed lengths:
W1: Ladder
W2: Ryan's stuff
W3: BL05 LCR
W4: BL05 LCR
W5: BL05 LCR
W6: BL05 LCR
W7: BL05 LCR
W8: BL05 LCR
W9: BL05 LCR
W10: BL05 LCR
W11: BL05 LCR
W12: BL05 LCR
W13: BL05 LCR
W14: BL05 LCR
Ladder
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