- Picked Colonies, Made Streak Plate, Grew Liquid Cultures
Seven colonies on backbone only plate.
Six colonies on negative control (cells and water only).
Many colonies on LCR experimental plates and traditional ligation experimental plates.
Picked three colonies per experimental sample.
Made Streak Plate
Picked three additional colonies per experimental plate to make streak colonies from. Stored at 4C.
Loaded picked colony and pipette tip into 14ml round bottom tube with 4mL LB Amp media.
Shook overnight at 37C and 244RPM.