Haynes Lab:Notebook/Engineering PC-TFs/2015/02/12

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Summary

Picking Colonies --> Liquid Cultures
No growth on negative control or BL09 lig2 plate. Will re-check concentration/purity of this plasmid.
~30 colonies for BL09 lig5 and BL01 LCR plate.

Picked three colonies from each plate and grew up in 4mL amp media. Incubated at 37°C and 250rpm overnight.