Haynes Lab:Notebook/Engineering PC-TFs/2014/12/02

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Summary

  • BL01, BL05, CMV/MV9 Purification AGAIN (insert purity unacceptable at last attempt).
Reagent Volume

Expected:
CMV/MV9 (Well 1): 5200
BL01 (Well 3): 2500, 3300
BL05 (Well 5): 2500, 3300


Image to Come

30 μL/lane; 1% agarose; Ladder

Observed lengths:
CMV/MV9 (Well 1): ~7000; linear so may not travel as far(?)
BL01 (Well 3): 2500, 3300
BL05 (Well 5): 2500, 3300

DNA(CMV/MV9, BL01, BL05) 15.0 μL
10X buffer 3.0 μL
XbaI 2.0 μL
SpeI (no SpeI for CMV/MV9) 2.0 μL
dH2O 8 (10 for CMV/MV9) μL
Total 30 μL --> 37°C/ 30 min.