Haynes Lab:Notebook/Engineering PC-TFs/2014/08/14

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Summary

  • Analyzed sequencing results.

Sequencing Results
Used ApE to open sequencing data from DNASU.
Order 9355 BL09rA and BL09rB (two colonies for BL09 which looked promising on the gel verification) were shown to have correct insertion of PcTF.
Matches occurred starting after the primer and continued onto the PcTF starting with the activation domain indicating successful forwards insertion.