Summary
Miniprepped the three colonies' liquid cultures
Eluted with 75μL dH2O.
Concentration
| Plasmid |
OD260 |
OD260/280 |
ng/μL
|
| 1. CMV/MV9 BL01 Ligation C1 |
0.17 |
1.899 |
170.143
|
| 1. CMV/MV9 BL01 Ligation C2 |
0.168 |
1.898 |
167.513
|
| 1. CMV/MV9 BL01 Ligation C3 |
0.201 |
1.885 |
200.8
|
Verification Strategy
Check with XbaI and NruI
Length of forward insertion.
|
Length of reverse insertion.
|
Length of no insertion.
|
More Strategy
Expected bp for; Forward insertion: 710, 7010; Reverse insertion: 3230, 4490; No insertion: 710, 4490.
|
Length of no insertion cut and forward insertion cut are the same, although vector/PcTF length would differ by the length of the PcTF. PCR verification with DD 123B and DD 122B should allow for verification as well.
|
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