Haynes Lab:Notebook/Engineering PC-TFs/2013/09/06

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September 6th, 2013 Bacterial Transformation of CMV Promoter Warm 100 μg/mL Amp agar plate at 37°C Thaw fresh tube of DH5α Turbo cells on ice Add .5 μL of DNA (CMV) to 10 μL dH2O in .5 mL tube Add 30 μL of DH5α Turbo Cells to DNA + dH2O Incubate cells + DNA on ice for 5 minutes. Label pre-warmed plate. Transfer cells + DNA onto agar. Add 8-10 glass beads, shake, then discard beads. Incubate plates at 37°C overnight and retrieve next day.