Haynes Lab:Notebook/Engineering PC-TFs/2013/09/06

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September 6th, 2013

Bacterial Transformation of CMV Promoter

  • Warm 100 μg/mL Amp agar plate at 37°C
  • Thaw fresh tube of DH5α Turbo cells on ice
  • Add .5 μL of DNA (CMV) to 10 μL dH2O in .5 mL tube
  • Add 30 μL of DH5α Turbo Cells to DNA + dH2O
  • Incubate cells + DNA on ice for 5 minutes.
  • Label pre-warmed plate.
  • Transfer cells + DNA onto agar.
  • Add 8-10 glass beads, shake, then discard beads.

Incubate plates at 37°C overnight and retrieve next day.