Haynes Lab:Notebook/Engineering PC-TFs/2012/10/19

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Summary

  • Retrieve gel slices from fridge.
  • Add 3 volumes of ADB Buffer to each volume of gel. (Each gel slice is assumed to be 200 mg)
  • Therefore, add 600 μL of ADB Buffer.
  • Incubate at 55°C for 10 minutes.
  • Load melted agarose solution into Spin Column in a collection tube.
  • Centrifuge at max speed for 30 seconds.
  • Discard flow-through.
  • Add 200 μL of DNA wash buffer to the spin columns and centrifuge for 30 seconds.
  • Place spin column in a 1.5 mL tube. Add 10μL of DNA Elution Buffer to elute the DNA.
  • Place tubes into freezer box.