Haynes Lab:Notebook/Engineering PC-TFs/2012/01/26

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Transformation

1. hPCD 2. flyPCD 3. fshPCD 4. no DNA

  • Warm 4 Amp agar plates (100 µg/mL) @ 37°C
  • 0.5 µL DNA + 10 µL dH2O (in 0.5 mL tubes)
  • Add 30 µL DH5 Turbo compotent cells to DNA + dH2O
  • Put immediately on ice --> incubate on ice for 5 min.
  • Label plates
  • Pipette cells + DNA onto agar --> Pour 10-15 beads onto the cells on the agar.
  • Shake the plates to spread the cells.
  • Invert. Dump dirty beads.
  • Incubate plates @ 37°C for 6-8 hours.