Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/08/06

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Haynes BioBrick Cloning Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Gel Electrophoresis

  1. BD004/MV2 a
  2. BD004/MV2 b
  3. BD006/MV2 a
  4. BD006/MV2 b


  • Cut with ApaLI (E/S insertion got rid of BB RFC23 cut sites)


Reagent Volume Expected:
* BD004/MV2 = 7108, 1243, 288
* MV2 only = 2998, 1243, 288
* BD006/MV2 = 7207, 1243, 288
Hover name
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 3.0 μL
10x buffer 1.5
ApaLI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.


  • Conclusions


  • Miniprep conc.'s
Sample 260/280 ng/μL
1. BD004/MV2 a 1.90 229.7
2. BD004/MV2 b 1.99 125.05
3. BD006/MV2 a 1.957 160.15
4. BD006/MV2 b 2.103 35.733