Plasmid Extraction and Validation
- BD004 MV2
- BD006 MV2
- Culture inoculation 8-4-15 (19 hour incubation)
- Culture 1-4: growth
- Culture 2-4: growth
- Culture 1-6: growth
- Culture 2-6: growth
- B0004 MV2
- Streak plate Colony 1: growth
- Streak plate colony 2: growth
- BD006 MV2
- Streak plate colony 1: growth
- Streak plate colony 2: growth
- Sample 1-4= B0004 MV2 colony 1
- OD260/280=1.90
- Concentration: 229.7 ng/μL
- Sample 2-4 = B0004 MV2 colony 2
- OD260/280=1.99
- Concentration: 125.05 ng/μL
- Sample 1-6 = B0006 MV2 colony 1
- OD260/280= 1.957
- Concentration: 160.15 ng/μL
- Sample 2-6= B0006 MV2 colony 2
- OD260/280 = 2.103
- Concentration: 35.733 ng/μL
- used 50 μL H2O for DNA elution
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Haynes BioBrick Cloning
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