Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/07/24

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Haynes BioBrick Cloning Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Plasmid Extraction and Validation

  • KAH126 MV2
  • Culture inoculation 7-23-15 (25 hour incubation)
    • Culture 1: growth
    • Culture 2: growth
    • Streak plate Colony 1: growth
    • Streak plate colony 2: growth
  • Sample 1= KAH126 MV2 colony 1
  • Sample 2 = KAH126 MV2 colony 2
    • Sample 1 : OD260/280=1.79
      • Concentration: 80 ng/μL
    • Sample 2: OD260/280=1.9
      • Concentration: 60 ng/μL

Gel Electrophoresis

  • 1% Agrose gel
    • 6μL sybr gel stain
  • Digests
    • DNA - 5.0μL
    • 10x FD buffer - 1.5μL
    • ECO RI - 1.0μL
    • Pst I - 1.0μL
    • dH2O - 6.5μL
  • Sample 1: DNA from colony 1
  • Sample 2: DNA from colony 2
    • Incubated at 37°C for 15 min
  • Electrophoresis
    • 1kbt ladder - 10μL
    • Sample 1 - 15μL
    • Sample 2 - 15μL
    • Gel ran for 45 min
Today's gel
15 μL/lane; 1% agarose; Ladder