Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/03

Haynes BioBrick Cloning

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Continuation of Restriction Digests and Gel Electrophoresis Restriction Digest Table Checked plasmid minipreps with EcoRI/PstI digests Reagent Volume Expected: 1. fshPCD = 186 bp, 4968 bp 15 μL/lane; 1% agarose; Ladder DNA(plasmid) 5.0 μL 10X FD Buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 6.5   15 μL --> 37°C/ 15 min. Conclusion There were faint bands at the 186 position; however, they are harder to see on the picture. The 4968 bands for all 3 are very visible. Fairly successful digest. DNA Sequencing Samples Submitted to DNASU on: 10/7/2013 Order Number: 8213 Plasmid 1 (fshPCD) - Forward P001 Plasmid 1 (fshPCD) - Reverse P002