Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/01

Haynes BioBrick Cloning

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Day 3- Minipreps fshPCD Liquid Culture Results NOTE: Cultures and Streaks taken out after 18 hours (37 incubator) and left on lab bench for 1 hour. fshPCD Cultures Innoculated 09/27/13 Cultures 1 and 2: Both show growth Streak 1 and 2: Both show growth Samples for Minipreps and Analysis Sample 1= fshPCD/V0120 colony 1; BBa_J176002; part= 186 bp; vector= 4968 bp Sample 2= fshPCD/V0120 colony 2; same DNA Concentration Data Plasmid OD260 OD260/280 ng/μL 1. fshPCD 1 0.014 1.714 24.682 2. fshPCD 2 0.013 1.762 28.523 Did not have good concentration, nothing else done after this point