Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/07/14

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07/14/2016

Ran a TypeIIs Cloning Protocol with pBta3 and Bta3R_MRV

Reagent Volume (uL)
Vector (100ng) 0.2
Insert (1:250 dilution) 2
10x FD 2
DTT 1
ATP 1
BbsI 1
Ligase (T4) 0.5
Water 12.3
Total 20