Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/07/13

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07/13/2016

Protocol for digest verification of LasR

Reagent Volume (uL)
DNA (1ug)
EcoRI 1
KpnI 1
10x Green Fast Digest Buffer 2
Water up t0 20uL

Incubate at 37°C for 10-15 minutes.

Run 10uL of the reaction on a gel. (Use small tooth combs).