Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/06/27

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

06/27/2016

In lab meeting, René suggested PCR-purifying the pLas insert after digestion, rather than heat inactivating. Since the insert is a small sequence, the high heat may not have annealed properly after denaturing.

I will go directly from PCR reaction to digestion with EcoRI and SpeI, and then PCR purify after a gel verification.

I will digest LasR_MRV with BbsI, heat inactivate and then PCR purify. (Instead of doing a gel extraction).