Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/06/27
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In lab meeting, René suggested PCR-purifying the pLas insert after digestion, rather than heat inactivating. Since the insert is a small sequence, the high heat may not have annealed properly after denaturing.
I will go directly from PCR reaction to digestion with EcoRI and SpeI, and then PCR purify after a gel verification.
I will digest LasR_MRV with BbsI, heat inactivate and then PCR purify. (Instead of doing a gel extraction).