Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/03/03

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Negative Receiver Plates

Despite the low concentration of DNA yielded from the gel extraction yesterday, the transformation was successful, as there were 5 colonies on the negative receiver plate and none on the water-only control, as shown in the picture below:

Figure 1 - Negative Receiver Plates
:Negative control receiver plates

MSV and Negative Receiver Liquid Cultures

We made a single liquid culture of the MSV (3 mL LB+Amp) and two liquid cultures of the negative receiver (2 mL LB+Amp each). The cultures were mini-prepped the following day.