Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/02/24

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Induction of BjaR and RhlR with RhlI Media

Cass and I took hourly measurements of the OD, GFP, and RFP of the BjaR and RhlR induced with RhlI media with the plate reader and the flow cytometer. After taking these measurements, we did not observe any induction. Cass suggests that this is possibly due to using an insufficient amount of inducer media.