Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/08/12

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08/12/2015

Ligate LasR_MRV with pLas

Trial 1 —
Ligate dephosphorylated LasR_MRV with pLas
[protocol]
Trial 2—
Ligate LasR_MRV with dephosphorylated pLas
[protocol]

Gel verify RhlI colony PCR and LasR

It looks like colony 9 and 15 are successful.
File:15.08.12 rhli and lasr gel verification.TIF.TIF
Backbone gel for reference—
File:Gel backbone only.tiff