Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/08/11

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Double digest pLas with EcoRI and SpeI for insertion.

I ran two reactions. One included rSAP and the other one did not
Incubate at 37°C for 30 minutes, then heat deactivate
80°C for 5 minutes, 65°C for 20 minutes