Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/08/04

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Gel-verify pLas PCR product.

Retry pLas + LasR_MRV ligation

  • Using newly PCR'd pLas and dephosphorylated LasR_MRV

Digest MSV with E, X, and rSAP, then heat deactivate

Digest PCR-purified RhlI with E, X, then heat deactivate