Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/08/03

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Digest pLas with EcoRI and SpeI

Treat LasR_MRV with Alkaline Phosphatase

Confirm completed LuxR and EsaR plasmids!

Only plasmids left are RhlI and LasR. Currently troubleshooting both and retrying ligations.