Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/21
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Ligase and no-ligase controls were created to help troubleshoot.The last LasR_MRV + pLas ligation was not successful (only one colony on plate, and 3 colonies on backbone plate). By doing two controls I hope to trace the problem: is the backbone ligating back to itself, or was it not fully digested?