Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/15
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Miniprep liquid cultures of pLux1, 2, 3 and LasR 1. Follow up with a XbaI + KpnI digestion; if there was an insertion, the KpnI site would be gone, resulting in one band. If there was no insertion, and the KpnI site was still there, there would be two bands.
Unsure why there are three bands for LasR1 and pLux1. pLux 2 and 3, however, look promising, so they will be sent in for sequencing.