Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/06/17

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Digest verification for senders that I mini-prepped yesterday looks promising: 15.06.19 senders digest verification.JPG copy.jpg

Two bands for CerI were expected since there are two EcoRI sites in the synthase. EsaI 2 and 3 looked like they were successful; these bands are longer than the others—since part of EsaI appeared to be truncated last time, the shorter bands probably represent the truncated plasmid.
I will send the following plasmids out for sequencing:
EsaI 2, EsaI 3, CerI 2, LasI 3, BjaI 3, BjaI 4