Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/22

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05/22/2015

We mini-prepped, double-digested, and gel-verified more colonies for the synthases (and receivers) that didn't work last time.

Sample Read# Location 260 Raw 280 Raw 320 Raw 260 280 260/280 ng/µL
1 B3 0.055 0.052 0.05 0 -0.001 0.286 -0.421
BraR 1 C2 0.36 0.224 0.073 0.3 0.157 1.912 300.277
BraR 2 C3 0.41 0.239 0.053 0.37 0.192 1.923 369.899
BjaI 6 D2 0.238 0.158 0.071 0.171 0.088 1.94 171.217
Bjal 7 D3 0.617 0.348 0.052 0.586 0.306 1.913 585.756
LasI 5 E2 0.385 0.224 0.047 0.35 0.182 1.923 349.716
LasI 6 E3 0.643 0.362 0.051 0.608 0.318 1.911 608.394
RhlI 3 F2 0.255 0.158 0.052 0.207 0.107 1.924 206.817
RhlI 4 F3 0.286 0.172 0.047 0.24 0.125 1.921 240.016