Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/19

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05/19/2015

We checked on the plates that were transformed yesterday (AubI and BjaI) and there were no colonies on the backbone or negative control plates using Ryan's NEB 10β cells! So I picked 3 colonies from each plate (shooting for ones that appeared red) and made 3mL liquid cultures. They are in the shaker. Colonies chosen are indicated on the plate.

I also fast-transformed the rest of the ligations (Bja, Cer, Esa, Las, Lux, Rpa, Sin) using NEB 10β cells. They are now in the incubator