Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/04/21

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04/21/2015

Digest verification of RpaI colony 5 showed that the plasmid is not what we think it is.

File:RpaI Gel Image New.jpg

We would expect to see an addition band at ~800bp in the digested lane if the cloning worked.



Next step is to retry with a new digestion with new alkaline phosphatase.