Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/04/16

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04/16/2015

Ran a colony PCR of the ligations. Picked 8 colonies per plate and focused on the bigger colonies to avoid picking backbone-only colonies (if the backbones ligated to another backbone, the resulting backbone would grow slower). Also ran a colony PCR on two colonies from the backbone plate.

Annealing temperature: 51°C
Extension time: 2minutes and 10 seconds (for a strand ~2200bp)