Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/04/01
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I checked the plates that were ligated yesterday, and there were colonies on the backbone control. There were no colonies on the negative control.
So, we decided to colony PCR the 9 ligations performed yesterday. We picked 8 colonies per plate to PCR.
We used the grid method to grow the colonies we picked for the PCR.