Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/17
|Project name||<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page|
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
10μL of DNA dilution buffer was then added to each tube, followed by 2μL(?) of ligation buffer and then finally, 1μL of ligase.
Rene: Pippet the reactions into an AMP agar plate, pour in ~8 glass beads and shake. Then store in 37C incubator overnight. Transfer to 4C fridge.