Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/06

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02/06/2015 I cut the PCR product of the insert from yesterday with BbsI and PstI. I used 5ul of the PCR reaction straight into the digestion: Thing vol in ul 1ug sample 5 H2O 11 10x buffer 2 PstI 1 BbsI 1 Total 20 I purified the digested insert using the Sigma PCR purification kit: [Insert Excel Concentration data here] I also ligated the inserts with backbones and transfected ____ cells with those plasmids. I had three plates: positive control, negative control, and a plate with the modular sender (MS). The positive control plate contained a known plasmid (EsaI). The negative control plate had no plasmid (?). The MS plate contained the ligated plasmid.