Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/03

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02/03/2015

I added enzymes to my insert sample (which was PCR-purified the day before) for digestion. I first added water to a 200μL PCR tube, then I added the sample, buffer, and two enzymes.


Restriction digest

Thing vol in ul
1ug sample 9.17
H2O 6.83
10x buffer 2
PstI 1
BbsI 1
Total 20


Purified with PCR clean up kit

Sample Read# 260 280 260/280 ng/µL
insert for ryan 0.012 0.007 1.62 12.1