Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/11/06

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mm/dd/yyyy Retried PCR of golden gate parts using Rene's primers, this time doing a Tm screen Reagent uL ddH2O 33 HF buffer 10 primer F (10uM) 2.5 primer R (10uM) 2.5 dNTP (10mM) 1 Template K084007 0.5 Phusion polymerase 0.5 Total 50 annealed for 30 seconds used Tm: 30, 32, 34, 36, 38 Number Template F Primer R Primer Annealing T Expected length 1 K084007 3/10/13 4-2-1 P069 P070 32 645 2 K084007 3/10/13 4-2-2 P069 P070 32 645 Still no clear bands observed for any of the annealing temperatures.