Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/19

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10:1 control plate has 0 colonies, 10:1 LuxR receiver plate has maybe 7 or so colonies (some are very small, and several of the plates have many colony-sized bubbles making it hard to count). Picked four colonies off X 10:1 plate to miniprep tomorrow.

Golden gate ligations for other receivers with 10:1 insert to bb ratio

Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl 0.74 0 0 0.5 1.25 2 5.51
Rhl Rec 0.74 2.78 1.68 0.5 1.25 2 1.05
Esa Rec 0.74 4.35 2.7 0.5 1.25 2 -1.54