Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15

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Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec 56.995 27 7.407407407


Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl 7.4 0 0 0.5 1.25 2 8.85
Lux Rec 7.4 2.94 2.25 0.5 1.25 2 3.66
Rhl Rec 7.4 2.78 1.68 0.5 1.25 2 4.39
Esa Rec 7.4 4.35 2.7 0.5 1.25 2 1.8