Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/06

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mm/dd/yyyy

Ryan set up PCR. Annealing temp 53, extension time 3:30min. Diluted each template down 1:1000.

Number Template F Primer R Primer Annealing T Expected length
1 pTet-mCh 2/14/14 74ng P127 P097 53 3148
2 LuxI sender const 8/31/13 179ng P063 P064 23 654
3 K084007 3/10/13 4-2-1 P069 P070 32 645
4 K084007 3/10/13 4-2-2 P069 P070 32 645
5 RBS-LasI 3/8/13 31ng P069 P070 32 645
6 RhlI sender const 9/27/13 72 ng P075 P076 39 642



Ryan ran gel of colony PCRs from last week.