Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/18

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Sequencing results:
Reverse primer didn't give results, turns out I removed the binding site during cloning.
Forward primer showed only backbone. Unsure what this means because the band size on the gel indicates the insert is there. Two possibilities: Backbone self-ligated or I moved the sequencing primer binding site to a new location.