Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/01

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04/01/2014 Sample Read# ng/µL fmol/ul ul Sample ul H2O I13522 P111 P12 38.172 25.15384615 7.951070336 2.048929664 G001 P105 P107 5.052 76.53846154 2.613065327 7.386934673 Thing Volume (ul) Part I 1 Part II 1 Part III 1 T5 ligase 1.25 BsmBI 0.5 T4 DNA ligase buffer 1 H2O 4.25 GG reaction Transformed into new DH5alphaT made by me soon after reaction finished. Long transformation, heat shock, 1.5 hour recovery time. New plates made by Behzad. Positive control plasmid, negative control of water.