Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/02/18

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02/18/2014

PCR of Senders for golden gate tomorrow.

Template Primer 1 Primer 2 Tm Approx length
pTet-mCh P089 P097 45 3000
LasI P069 P070 32 650
RhlI P075 P076 39 650


Extension time 3 minutes, annealing temp 46.