Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/02/14

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02/14/2014

Miniprep cultures picked yesterday. Golden gate seems to have been successful because the liquid cultures were clearly red. This new part is pTet-B0034-mCh-T-T in pSB1A3. This will be used to build new senders.

Sample Read# 260 280 260/280 ng/µL
pTet-B0034-mCh-T-T in pSB1A3 5-1 0.108 0.056 1.927 108.225
pTet-B0034-mCh-T-T in pSB1A3 6-2 0.077 0.039 1.986 76.508
pTet-B0034-mCh-T-T in pSB1A3 6-1 0.074 0.036 2.058 74.252
pTet-B0034-mCh-T-T in pSB1A3 5-2 0.102 0.05 2.021 101.873



Prepare K12 and DH5αT competent cells.