Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/19

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09/19/2013 RhlI OD650 GFP in LB pH 0.612 9929 7-8 EsaI OD650 GFP in LB pH 0.551 257 7-8 Double checked super weak EsaI GFP expression and it's correct. Pelleted cells were not as green as RhlI. F2620-RFP OD650 mCherry in LB 0.544 668 F2620-GFP OD650 GFP in LB 0.502 1166 Control supernatant was DH5αT K092900-E0420 which was not a perfect control. pH was 8-9. 11:40am measured mCherry Ex:572/Em:607 Spun Rhl and Esa at 10:30am. Moved 1mL to round bottom tubes. Added 40uL LuxR-GFP or LuxR-RFP. Added 1ul amp. RhlI Sender Receiver RhlI LuxR-GFP EsaI LuxR-GFP Ctrl LuxR-GFP RhlI LuxR-RFP EsaI LuxR-RFP Ctrl LuxR-RFP Thawed 1mL supernatant tubes, transferred to round bottom tubes. Added 40uL LuxR-GFP. Added 1uL amp. Sender Receiver RhlI 1 LuxR-GFP RhlI 2 LuxR-GFP RhlI 3 LuxR-GFP minipreps picked 9/18/13 Part Result K081002 grew slowly I13522-3 grew slowly K092900-E0420 (80.5) didn't grow F2620-GFP grew RhlI grew F2620-RFP grew K081020 grew EsaI grew K092900-E0420 (80) grew pretty sure i put these in the freezer and did them another day.