Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/02/27

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Entry title

Picked 2 colonies from each plate and grew in 3mls of LB + amp

Part number Description Part size (bp) Backbone
F2620 p(tetR)-RBS-luxR-Stop-lux pR 1061 pSB1A2
K082035 p(tetR)-RBS-rhlI-Stop-Stop 884 pSB1A2
R0071 RhlR-activated promoter 53 pSB1A2
K084007 LacI-RBS-LasI 835 pSB1AK3
K081016 RBS-lasI-Stop 735 pSB1AK3
K081023 RBS-cl lam-RBS-lasR-Stop 1653 pSB1AK3