Haynes Lab:Notebook/CRISPR Editing/2016/09/02

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09/02/2016 qPCR of Luc14 31 and 34 treated ChIP samples triplicate, IgG, FLAG, singlet inputs, technical triplicates, 313/311, GAPDH Totally worked! Small error bars! Started ChIPs on Gal4+puro and Gal4+dox g031 and g034 preclear with beads for 3 hours, nutation, 4deg add FLAG beads cut ends of tips to make wide bore 20ul beads/IP wash 3x with TBS buffer resuspend to original volume add 20ul to empty tubes put in magnet rack, remove TBS add samples add IgG to IgG samples nutate overnight at 4deg removed dox from Gal4+dox cells, expanded all cells to T-175s